Converting a breast cancer microarray signature into a high-throughput diagnostic test.

Publication Name: BMC Genomics

Author(s): Glas AM, Floore A, Delahaye LJMJ et al.

Background: A 70-gene tumor expression profile was established as a powerful predictor of disease outcome in young breast cancer patients. This profile, however, was generated on microarrays containing 25,000 60-mer oligonucleotides that are not designed for processing of many samples … Continued

Mammary gland biology and breast cancer. Conference on Common Molecular Mechanisms of Mammary Gland Development and Breast Cancer Progression

Publication Name: EMBO Reports

Author(s): Sharon F McGee, Fiona Lanigan, Emer Gilligan,and Bernd Groner

Introduction:  As a model system for biological investigations, the mouse mammary gland is as versatile as the Drosophilaeye. This comparison does not reflect any ignorance of comparative anatomy, but rather it highlights the multitude of possibilities that the mammary gland … Continued

Validation and Clinical Utility of a 70-Gene Prognostic Signature for Women With Node-Negative Breast Cancer

Publication Name: Journal of the National Cancer Institute

Author(s): Marc Buyse, Sherene Loi, Laura van’t Veer, Giuseppe Viale, Mauro Delorenzi, Annuska M. Glas, Mahasti Saghatchian d’Assignies, Jonas Bergh, Rosette Lidereau, Paul Ellis, Adrian Harris, Jan Bogaerts, Patrick Therasse, Arno Floore, Mohamed Amakrane, Fanny Piette, Emiel Rutgers, Christos Sotiriou, Fatima Cardoso, Martine J. Piccart

Background: A 70-gene signature was previously shown to have prognostic value in patients with node-negative breast cancer. Our goal was to validate the signature in an independent group of patients. Methods: Patients (n = 307, with 137 events after a … Continued

Frequency and Cost of Chemotherapy-Related Serious Adverse Effects in a Population Sample of Women With Breast Cancer

Publication Name: Journal of the National Cancer Institute

Author(s): Michael J. Hassett , A. James O’Malley , Juliana R. Pakes , Joseph P. Newhouse , Craig C. Earle

Background: The number, nature, and costs of serious adverse effects experienced by younger women receiving chemotherapy for breast cancer outside of clinical trials are unknown. Methods: From a database of medical claims made by individuals with employer-provided health insurance between … Continued

Microarray analysis and tumor classification.

Publication Name: New England Journal of Medicine

Author(s): J. Quackenbush, Ph.D.

DNA microarray analysis was first described in the mid-1990s as a means to probe the expression of thousands of genes simultaneously(1,2) and was quickly adopted by the research community for the study of a wide range of biologic processes. Most … Continued

Concordance among Gene-Expression– Based Predictors for Breast Cancer

Publication Name: The New England Journal of Medicine

Author(s): Cheng Fan, M.S., Daniel S. Oh, Ph.D., Lodewyk Wessels, Ph.D., Britta Weigelt, Ph.D., Dimitry S.A. Nuyten, M.D., Andrew B. Nobel, Ph.D., Laura J. van’t Veer, Ph.D., and Charles M. Perou, Ph.D.

Background Gene expression profiling studies of primary breast tumors performed by different laboratories have resulted in the identification of a number of distinct prognostic profiles, or gene sets, with little overlap in terms of gene identity. Methods To compare the … Continued

Molecular Portraits and 70-Gene Prognosis Signature Are Preserved throughout the Metastatic Process of Breast Cancer

Publication Name: The Journal of Cancer Research

Author(s): Britta Weigelt, Zhiyuan Hu, Xiaping He, Chad Livasy, Lisa A. Carey, Matthew G. Ewend, Annuska M. Glas, Charles M. Perou and Laura J. van't Veer

Microarray analysis has been shown to improve risk stratification of breast cancer. Breast tumors analyzed by hierarchical clustering of expression patterns of “intrinsic” genes have been reported to subdivide into at least four molecular subtypes that are associated with distinct … Continued

Molecular profiling of breast cancer: clinical implications

Publication Name: British Journal of Cancer

Author(s): S Cleator, A Ashworth

Breast cancers are routinely subcategorised on the basis of clinical stage, cellular morphology and immunohistochemical analysis of a small number of markers. The recent development of gene expression microarray and related technologies provides an opportunity to perform more detailed profiling … Continued

A Gene-Expression Signature as a Predictor of Survival in Breast Cancer.

Publication Name: New England Journal of Medicine

Author(s): Marc J. van de Vijver, M.D., Ph.D., Yudong D. He, Ph.D., Laura J. van 't Veer, Ph.D., Hongyue Dai, Ph.D., Augustinus A.M. Hart, M.Sc., Dorien W. Voskuil, Ph.D., George J. Schreiber, M.Sc., Johannes L. Peterse, M.D., Chris Roberts, Ph.D., Matthew J. Marton, Ph.D., Mark Parrish, Douwe Atsma, Anke Witteveen, Annuska Glas, Ph.D., Leonie Delahaye, Tony van der Velde, Harry Bartelink, M.D., Ph.D., Sjoerd Rodenhuis, M.D., Ph.D., Emiel T. Rutgers, M.D., Ph.D., Stephen H. Friend, M.D., Ph.D., and René Bernards, Ph.D.

Background: A more accurate means of prognostication in breast cancer will improve the selection of patients for adjuvant systemic therapy. Methods: Using microarray analysis to evaluate our previously established 70-gene prognosis profile, we classified a series of 295 consecutive patients … Continued

Gene expression profiling predicts clinical outcome of breast cancer

Publication Name: Nature

Author(s): Laura J. van 't Veer, Hongyue Dai, Marc J. van de Vijver, Yudong D. He, Augustinus A. M. Hart, Mao Mao, Hans L. Peterse, Karin van der Kooy, Matthew J. Marton, Anke T. Witteveen, George J. Schreiber, Ron M. Kerkhoven, Chris Roberts, Peter S. Linsley, René Bernards & Stephen H. Friend

Breast cancer patients with the same stage of disease can have markedly different treatment responses and overall outcome. The strongest predictors for metastases (for example, lymph node status and histological grade) fail to classify accurately breast tumors according to their … Continued