Kit Contents

The MammaPrint BluePrint Kit consists of four boxes that include the different reagents required for library preparation and target enrichment of up to 16 samples prior to sequencing. Each box has storage temperature requirements specific to its contents.

MammaPrint BluePrint NGS RNA Library Prep
(Pre-PCR)

Storage: -20°C

Box 1 of 4

Agendia NGS Fragmentation Mix
Agendia NGS 1st Strand Master Mix
Agendia NGS 2nd Strand + End Repair Enzyme Mix
Agendia NGS 2nd Strand + End Repair Oligo Mix
Agendia NGS dA Tailing MM
Agendia NGS Oligo Adaptor Mix
Agendia NGS Ligation Master Mix
Agendia NGS Forward PCR Primer
Agendia NGS PCR Master Mix
Agendia NGS Uracil DNA Glycosylase (UDG)
Agendia NGS Reverse PCR Primer
Agendia NGS Nuclease-Free Water

MammaPrint BluePrint NGS Target Enrichment
(Post-PCR Box 1)

Storage: Room Temp

Box 2 of 4

Agendia NGS Hyb 1
Agendia NGS Hyb 2
Agendia NGS Hyb 4
Agendia NGS Binding Buffer
Agendia NGS Wash Buffer 1
Agendia NGS Wash Buffer 2
Agendia NGS Nuclease-Free Water
Agendia NGS Elution Buffer
Agendia NGS Neutralization Buffer

MammaPrint BluePrint NGS Target Enrichment
(Post-PCR Box 2)

Storage: -20°C

Box 3 of 4

Agendia NGS Indexing Block 1
Agendia NGS Block 2
Agendia NGS ILMN Indexing Block 3
Agendia NGS RNase Block
Agendia NGS Hyb 3
Agendia NGS Post-Capture PCR Primer
Agendia NGS PCR Master Mix
Agendia NGS 8bp Index Plate

MammaPrint BluePrint NGS Panel
 
Storage: -80°C

Box 4 of 4

MammaPrint BluePrint Baits Library
For a list of equipment and reagents required but not provided with the Kit, please consult the Package Insert, found on the Resources page.